| Fish | Lake Sturgeon (Northwestern Ontario population) | Acipenser fulvescens | THR | range |
| Fish | Lake Sturgeon (Southern Hudson Bay/James Bay population) | Acipenser fulvescens | SC | range |
| Fish | Lake Sturgeon (Upper Great Lakes/St. Lawrence population) | Acipenser fulvescens | THR | range |
| Amphibians | Northern Cricket Frog | Acris crepitans | END | range |
| Plants | Gattinger’s Agalinis | Agalinis gattingeri | END | range |
| Plants | Skinner’s Agalinis | Agalinis skinneriana | END | range |
| Plants | Colicroot | Aletris farinosa | THR | range |
| Amphibians | Jefferson Salamander [PDF fact sheet] | Ambystoma jeffersonianum | THR | range |
| Amphibians | Small-mouthed Salamander | Ambystoma texanum | END | range |
| Amphibians | Eastern Tiger Salamander | Ambystoma tigrinum | EXP | range |
| Plants | Scarlet Ammannia | Ammannia robusta | END | range |
| Fish | Eastern Sand Darter | Ammocrypta pellucida | THR | range |
| Birds | Henslow’s Sparrow | Ammodramus henslowii | END | range |
| Amphibians | Fowler’s Toad | Anaxyrus fowleri | THR | range |
| Fish | American Eel | Anguilla rostrata | END | range |
| Reptiles | Spiny Softshell | Apalone spinifera | THR | range |
| Birds | Golden Eagle | Aquila chrysaetos | END | range |
| Plants | Green Dragon | Arisaema dracontium | SC | range |
| Plants | Forked Three-awned Grass | Aristida basiramea | END | range |
| Plants | Tuberous Indian-plantain | Arnoglossum plantagineum | SC | range |
| Birds | Short-eared Owl | Asio flammeus | SC | range |
| Plants | Hart’s-tongue Fern | Asplenium scolopendrium americanum | SC | range |
| Plants | Branched Bartonia | Bartonia paniculata | THR | range |
| Plants | Cherry Birch | Betula lenta | END | range |
| Mosses | Spoon-leaved Moss | Bryoandersonia illecebra | END | range |
| Plants | Bluehearts | Buchnera americana | END | range |
| Birds | Red Knot rufa subspecies | Calidris canutus rufa | END | range |
| Insects | Frosted Elfin | Callophrys irus | EXP | range |
| Plants | Wild Hyacinth | Camassia scilloides | THR | range |
| Mammals | Eastern Wolf | Canis lupus lycaon | SC | range |
| Birds | Whip-poor-will [PDF fact sheet] | Caprimlugus vociferus | THR | |
| Plants | Juniper Sedge | Carex juniperorum | END | range |
| Plants | False Hop Sedge | Carex lupuliformis | END | range |
| Plants | American Chestnut | Castanea dentata | END | range |
| Plants | Dwarf Hackberry | Celtis tenuifolia | THR | range |
| Birds | Chimney swift [PDF fact sheet] | Chaetura pelagica | THR | |
| Birds | Piping Plover | Charadrius melodus | END | range |
| Reptiles | Snapping turtle [PDF fact sheet] | Chelydra serpentina | SC | |
| Plants | Spotted Wintergreen | Chimaphila maculata | END | range |
| Birds | Black Tern | Chlidonias niger | SC | range |
| Birds | Common nighthawk [PDF fact sheet] | Chordeiles minor | SC | |
| Plants | Hill’s Thistle | Cirsium hillii | THR | range |
| Plants | Pitcher’s Thistle | Cirsium pitcheri | END | range |
| Reptiles | Spotted Turtle | Clemmys guttata | END | range |
| Fish | Redside Dace | Clinostomus elongatus | END | range |
| Birds | Northern Bobwhite | Colinus virginianus | END | range |
| Plants | Spring Blue-eyed Mary | Collinsia verna | EXP | range |
| Reptiles | Blue Racer | Coluber constrictor foxii | END | range |
| Birds | Olive-sided flycatcher [PDF fact sheet] | Contopus cooperi | SC | |
| Fish | Upper Great Lakes Kiyi | Coregonus kiyi kiyi | SC | range |
| Fish | Shortnose Cisco | Coregonus reighardi | END | range |
| Fish | Shortjaw Cisco | Coregonus zenithicus | THR | range |
| Plants | Eastern Flowering Dogwood | Cornus florida | END | range |
| Birds | Yellow Rail | Coturnicops noveboracensis | SC | range |
| Reptiles | Timber Rattlesnake | Crotalus horridus | EXP | range |
| Plants | Small White Lady’s-slipper | Cypripedium candidum | END | range |
| Insects | Monarch | Danaus plexippus | SC | range |
| Mammals | Beluga | Delphinapterus leucas | SC | range |
| Birds | Cerulean Warbler | Dendroica cerulea | SC | range |
| Birds | Kirtland’s Warbler | Dendroica kirtlandii | END | range |
| Plants | Illinois Tick-trefoil | Desmodium illinoense | EXP | range |
| Amphibians | Northern Dusky Salamander | Desmognathus fuscus | END | range |
| Amphibians | Allegheny Mountain Dusky Salamander | Desmognathus ochrophaeus | END | range |
| Plants | Horsetail Spike-rush | Eleocharis equisetoides | END | range |
| Plants | Bent spike-rush [PDF fact sheet] | Eleocharis geniculata | END | |
| Birds | Acadian Flycatcher | Empidonax virescens | END | range |
| Reptiles | Blanding’s Turtle | Emydoidea blandingii | THR | range |
| Plants | False Rue-anemone | Enemion biternatum | THR | range |
| Molluscs | Northern Riffleshell | Epioblasma torulosa rangiana | END | range |
| Molluscs | Snuffbox | Epioblasma triquetra | END | range |
| Fish | Gravel Chub | Erimystax x-punctatus | EXP | range |
| Fish | Lake Chubsucker | Erimyzon sucetta | THR | range |
| Insects | Eastern Persius Duskywing | Erynnis persius persius | EXP | range |
| Fish | Grass Pickerel | Esox americanus vermiculatus | SC | range |
| Plants | White Wood Aster | Eurybia divaricata | THR | range |
| Fish | Cutlip Minnow | Exoglossum maxillingua | THR | range |
| Birds | Peregrine Falcon [PDF fact sheet] | Falco peregrinus | THR | range |
| Mosses | Pygmy Pocket Moss | Fissidens exilis | SC | range |
| Plants | American Columbo | Frasera caroliniensis | END | range |
| Plants | Blue Ash | Fraxinus quadrangulata | SC | range |
| Fish | Blackstripe Topminnow | Fundulus notatus | SC | range |
| Plants | White Prairie Gentian | Gentiana alba | END | range |
| Reptiles | Wood Turtle [PDF fact sheet] | Glyptemys insculpta | END | range |
| Insects | Rapids clubtail [PDF fact sheet] | Gomphus quadricolor | END | |
| Reptiles | Northern Map Turtle | Graptemys geographica | SC | range |
| Mammals | Wolverine | Gulo gulo | THR | range |
| Plants | Kentucky Coffee-tree | Gymnocladus dioicus | THR | range |
| Amphibians | Spring Salamander | Gyrinophilus porphyriticus | EXP | range |
| Birds | Bald Eagle | Haliaeetus leucocephalus | SC | range |
| Reptiles | Eastern Hog-nosed Snake | Heterodon platirhinos | THR | range |
| Plants | Swamp Rose-mallow | Hibiscus moscheutos | SC | range |
| Plants | Goldenseal | Hydrastis canadensis | THR | range |
| Plants | Lakeside Daisy | Hymenoxys herbacea | THR | range |
| Fish | Northern Brook Lamprey | Ichthyomyzon fossor | SC | range |
| Birds | Yellow-breasted Chat | Icteria virens | SC | range |
| Plants | Dwarf Lake Iris | Iris lacustris | THR | range |
| Plants | Engelmann’s Quillwort [PDF fact sheet] | Isoetes engelmannii | END | range |
| Plants | Small Whorled Pogonia | Isotria medeoloides | END | range |
| Plants | Large Whorled Pogonia | Isotria verticillata | END | range |
| Birds | Least Bittern | Ixobrychus exilis | THR | range |
| Plants | Butternut | Juglans cinerea | END | range |
| Plants | American Water-willow | Justicia americana | THR | range |
| Reptiles | Milksnake | Lampropeltis triangulum | SC | range |
| Molluscs | Wavy-rayed Lampmussel | Lampsilis fasciola | END | range |
| Birds | Loggerhead Shrike | Lanius ludovicianus | END | range |
| Fish | Spotted Gar | Lepisosteus oculatus | THR | range |
| Fish | Warmouth | Lepomis gulosus | SC | range |
| Lichens | Flooded Jellyskin | Leptogium rivulare | THR | range |
| Plants | Slender Bush-clover (Tallgrass Prairie Heritage Park, Ojibway Park and Black Oak Heritage Park in the City of Windsor) | Lespedeza virginica | END | range |
| Plants | Dense Blazing Star | Liatris spicata | THR | range |
| Molluscs | Eastern Pondmussel | Ligumia nasuta | END | range |
| Plants | Purple Twayblade | Liparis liliifolia | END | range |
| Plants | Small-flowered Lipocarpha | Lipocarpha micrantha | THR | range |
| Insects | Karner Blue | Lycaeides melissa samuelis | EXP | range |
| Fish | Silver Chub | Macrhybopsis storeriana | SC | range |
| Plants | Cucumber Tree | Magnolia acuminata | END | range |
| Birds | Red-headed Woodpecker | Melanerpes erythrocephalus | SC | range |
| Mammals | Woodland Vole | Microtus pinetorum | SC | range |
| Fish | Spotted Sucker | Minytrema melanops | SC | range |
| Plants | Red Mulberry | Morus rubra | END | range |
| Fish | River Redhorse | Moxostoma carinatum | SC | range |
| Fish | Black Redhorse | Moxostoma duquesnei | THR | range |
| Reptiles | Lake Erie Watersnake | Nerodia sipedon insularum | END | range |
| Fish | Pugnose Shiner | Notropis anogenus | END | range |
| Fish | Bridle Shiner | Notropis bifrenatus | SC | range |
| Fish | Silver Shiner | Notropis photogenis | SC | range |
| Fish | Northern Madtom | Noturus stigmosus | END | range |
| Birds | Eskimo Curlew | Numenius borealis | END | range |
| Molluscs | Round Hickorynut | Obovaria subrotunda | END | range |
| Fish | Pugnose Minnow | Opsopoeodus emiliae | SC | range |
| Plants | Eastern Prickly Pear Cactus (Fish Point Provincial Nature Reserve on Pelee Island) | Opuntia humifusa | END | range |
| Plants | American Ginseng | Panax quinquefolius | END | range |
| Reptiles | Eastern Foxsnake (Carolinian population) | Pantherophis gloydi | END | range |
| Reptiles | Eastern Foxsnake (Georgian Bay population) | Pantherophis gloydi | THR | range |
| Reptiles | Gray Ratsnake (Carolinian population) | Pantherophis spiloides | END | range |
| Reptiles | Gray Ratsnake (Frontenac axis population) | Pantherophis spiloides | THR | range |
| Insects | Aweme Borer Moth | Papaipema aweme | END | range |
| Birds | American White Pelican | Pelecanus erythrorhynchos | THR | range |
| Fish | Channel Darter | Percina copelandi | THR | range |
| Plants | Broad Beech Fern | Phegopteris hexagonoptera | SC | range |
| Insects | West Virginia White | Pieris virginiensis | SC | range |
| Plants | Heart-leaved Plantain | Plantago cordata | END | range |
| Plants | Eastern Prairie Fringed-orchid [PDF fact sheet] | Platanthera leucophaea | END | range |
| Reptiles | Common Five-lined Skink (Carolinian population) | Plestiodon fasciatus | END | range |
| Reptiles | Common Five-lined Skink (Southern Shield population) | Plestiodon fasciatus | SC | range |
| Molluscs | Round Pigtoe | Pleurobema sintoxia | END | range |
| Birds | Horned grebe [PDF fact sheet] | Podiceps auritus | SC | |
| Plants | Pink Milkwort | Polygala incarnata | END | range |
| Fish | Paddlefish | Polyodon spathula | EXP | range |
| Plants | Hill’s Pondweed | Potamogeton hillii | SC | range |
| Plants | Ogden's Pondweed | Potamogeton ogdenii | END | range |
| Birds | Prothonotary Warbler | Protonotaria citrea | END | range |
| Plants | Common Hoptree | Ptelea trifoliata | THR | range |
| Molluscs | Kidneyshell | Ptychobranchus fasciolaris | END | range |
| Mosses | Incurved Grizzled Moss | Ptychomitrium incurvum | EXP | range |
| Mammals | Mountain Lion or Cougar | Puma concolor | END | range |
| Plants | Hoary Mountain-mint | Pycnanthemum incanum | END | range |
| Molluscs | Mapleleaf Mussel | Quadrula quadrula | THR | range |
| Plants | Shumard Oak | Quercus shumardii | SC | range |
| Birds | King Rail | Rallus elegans | END | range |
| Mammals | Woodland Caribou (Forest-dwelling boreal population) | Rangifer tarandus caribou | THR | range |
| Reptiles | Queensnake | Regina septemvittata | THR | range |
| Plants | Climbing Prairie Rose | Rosa setigera | SC | range |
| Plants | Toothcup | Rotala ramosior | END | range |
| Fish | Atlantic Salmon (Lake Ontario population) | Salmo salar | EXP | range |
| Fish | Aurora Trout | Salvelinus fontinalis timagamiensis | END | range |
| Mammals | Eastern Mole | Scalopus aquaticus | SC | range |
| Birds | Louisiana Waterthrush | Seiurus motacilla | SC | range |
| Molluscs | Mudpuppy Mussel | Simpsonaias ambigua | END | range |
| Reptiles | Massasauga | Sistrurus catenatus | THR | range |
| Plants | Round-leaved Greenbrier | Smilax rotundifolia | THR | range |
| Plants | Houghton’s Goldenrod | Solidago houghtonii | THR | range |
| Plants | Riddell’s Goldenrod | Solidago riddellii | SC | range |
| Plants | Showy Goldenrod | Solidago speciosa | END | range |
| Reptiles | Eastern Musk Turtle | Sternotherus odoratus | THR | range |
| Plants | Wood-poppy | Stylophorum diphyllum | END | range |
| Plants | Willowleaf Aster | Symphyotrichum praealtum | THR | range |
| Plants | Crooked-stem Aster | Symphyotrichum prenanthoides | THR | range |
| Plants | Western Silvery Aster [PDF fact sheet] | Symphyotrichum sericeum | END | range |
| Mammals | American Badger [PDF fact sheet] | Taxidea taxus | END | range |
| Plants | Virginia Goat’s-rue | Tephrosia virginiana | END | range |
| Reptiles | Butler’s Gartersnake | Thamnophis butleri | THR | range |
| Reptiles | Eastern Ribbonsnake | Thamnophis sauritus | SC | range |
| Plants | Few-flowered Club-rush (Pickering and Hamilton) [PDF fact sheet] | Trichophorum planifolium | END | range |
| Plants | Drooping Trillium | Trillium flexipes | END | range |
| Plants | Nodding Pogonia | Triphora trianthophora | END | range |
| Molluscs | Fawnsfoot [PDF fact sheet] | Truncilla donaciformis | END | |
| Birds | Greater Prairie-Chicken | Tympanuchus cupido | EXP | range |
| Birds | Barn Owl [PDF fact sheet] | Tyto alba | END | range |
| Mammals | Grey Fox | Urocyon cinereoargenteus | THR | range |
| Mammals | Polar Bear | Ursus maritimus | THR | range |
| Plants | Deerberry | Vaccinium stamineum | THR | range |
| Birds | Golden-winged Warbler | Vermivora chrysoptera | SC | range |
| Molluscs | Rayed Bean | Villosa fabalis | END | range |
| Molluscs | Rainbow Mussel | Villosa iris | THR | range |
| Plants | Bird’s-foot Violet | Viola pedata | END | range |
| Birds | Canada warbler [PDF fact sheet] | Wilsonia canadensis | SC | |
| Birds | Hooded Warbler | Wilsonia citrina | SC | range |
| Plants | Blunt-lobed Woodsia | Woodsia obtusa | END | range |